Plasmid_Backbone

Part:BBa_K1559011:Design

Designed by: Xiuqi (Rex) Xia   Group: iGEM14_Toronto   (2014-10-14)


Low-copy plasmid pTRC99A (trc promoter and lacI removed)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2563
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2569
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2563
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2563
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2563
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2578
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 156
    Illegal BsaI site found at 1224
    Illegal SapI.rc site found at 2306


Design Notes

PCR amplify 376 to 2899 from original pTRC99A sequence (see link in sources). Then add biobrick sites using primer extension.

Source

Sequence and annotation:

http://www.addgene.org/vector-database/4402/

References